[Inhibition of autophagy initiation stage enhances camptothecin-induced apoptosis in NCI-H1975 cells].
Identifieur interne : 000C84 ( Main/Exploration ); précédent : 000C83; suivant : 000C85[Inhibition of autophagy initiation stage enhances camptothecin-induced apoptosis in NCI-H1975 cells].
Auteurs : Yaping Zhang [République populaire de Chine] ; Li Cao [République populaire de Chine] ; Qiang Su [République populaire de Chine] ; Kai Cheng [République populaire de Chine] ; Xiaoyan Zhang [Oman]Source :
- Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology [ 1007-8738 ] ; 2017.
Descripteurs français
- KwdFr :
- Adénine (analogues et dérivés), Adénine (pharmacologie), Apoptose (), Autophagie (), Camptothécine (pharmacologie), Carcinome pulmonaire non à petites cellules (anatomopathologie), Carcinome pulmonaire non à petites cellules (traitement médicamenteux), Chloroquine (pharmacologie), Facteur de croissance transformant bêta-1 (analyse), Humains, Lignée cellulaire tumorale, Prolifération cellulaire (), Tumeurs du poumon (anatomopathologie), Tumeurs du poumon (traitement médicamenteux).
- MESH :
- analogues et dérivés : Adénine.
- analyse : Facteur de croissance transformant bêta-1.
- anatomopathologie : Carcinome pulmonaire non à petites cellules, Tumeurs du poumon.
- pharmacologie : Adénine, Camptothécine, Chloroquine.
- traitement médicamenteux : Carcinome pulmonaire non à petites cellules, Tumeurs du poumon.
- Apoptose, Autophagie, Humains, Lignée cellulaire tumorale, Prolifération cellulaire.
English descriptors
- KwdEn :
- Adenine (analogs & derivatives), Adenine (pharmacology), Apoptosis (drug effects), Autophagy (drug effects), Camptothecin (pharmacology), Carcinoma, Non-Small-Cell Lung (drug therapy), Carcinoma, Non-Small-Cell Lung (pathology), Cell Line, Tumor, Cell Proliferation (drug effects), Chloroquine (pharmacology), Humans, Lung Neoplasms (drug therapy), Lung Neoplasms (pathology), Transforming Growth Factor beta1 (analysis).
- MESH :
- chemical , analogs & derivatives : Adenine.
- chemical , analysis : Transforming Growth Factor beta1.
- chemical , pharmacology : Adenine, Camptothecin, Chloroquine.
- drug effects : Apoptosis, Autophagy, Cell Proliferation.
- drug therapy : Carcinoma, Non-Small-Cell Lung, Lung Neoplasms.
- pathology : Carcinoma, Non-Small-Cell Lung, Lung Neoplasms.
- Cell Line, Tumor, Humans.
Abstract
Objective To explore the effect of autophagic inhibitors chloroquine (CQ) and 3-methyl adenine (3-MA) on apoptosis of non-small cell lung adenocarcinoma NCI-H1975 cells induced by camptothecin (CPT). Methods After NCI-H1975 cells were treated with CPT, cell proliferation was detected by CCK-8 assay, morphological changes of cells were observed by PI staining, and the apoptosis of NCI-H1975 cells was determined by flow cytometry. The levels of autophagy-and apoptosis-related proteins LC3I, LC3II, P62, caspase-3 and poly ADP-ribose polymerase (PARP) and transforming growth factor beta 1 (TGF-beta 1) were detected by Western blot analysis. Results After CPT treatment, the ratio of LC3II to LC3I was raised. The apoptotic protease caspase-3 and substrate PARP were obviously degraded, which could be enhanced by 3-MA but inhibited by CQ. It was also found that the intracellular TGF-beta 1 was reduced after CPT treatment. Conclusion Inhibition of autophagy initiation stage in NCI-H1975 cells can increase the sensitivity of cell apoptosis induced by CPT.
PubMed: 29382424
Affiliations:
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Shanxi Medical University, First Affiliated Hospital of Shanxi Medical University, Taiyuan 030001, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Medical Laboratory Science, Fenyang College of Shanxi Medical University, Fenyang 032200; Shanxi Medical University, First Affiliated Hospital of Shanxi Medical University, Taiyuan 030001</wicri:regionArea><wicri:noRegion>Taiyuan 030001</wicri:noRegion></affiliation></author><author><name sortKey="Su, Qiang" sort="Su, Qiang" uniqKey="Su Q" first="Qiang" last="Su">Qiang Su</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Neurology, First Affiliated Hospital of Shanxi Medical University, Taiyuan 030001, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Neurology, First Affiliated Hospital of Shanxi Medical University, Taiyuan 030001</wicri:regionArea><wicri:noRegion>Taiyuan 030001</wicri:noRegion></affiliation></author><author><name sortKey="Cheng, Kai" sort="Cheng, Kai" uniqKey="Cheng K" first="Kai" last="Cheng">Kai Cheng</name><affiliation wicri:level="1"><nlm:affiliation>Department of Medical Laboratory Science, Fenyang College of Shanxi Medical University, Fenyang 032200, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Medical Laboratory Science, Fenyang College of Shanxi Medical University, Fenyang 032200</wicri:regionArea><wicri:noRegion>Fenyang 032200</wicri:noRegion></affiliation></author><author><name sortKey="Zhang, Xiaoyan" sort="Zhang, Xiaoyan" uniqKey="Zhang X" first="Xiaoyan" last="Zhang">Xiaoyan Zhang</name><affiliation wicri:level="1"><nlm:affiliation>Department of Medical Laboratory Science, Fenyang College of Shanxi Medical University, Fenyang 032200; Shanxi Medical University, First Affiliated Hospital of Shanxi Medical University, Taiyuan 030001, China. *Corresponding author, E-mail: 931326464@qq.com.</nlm:affiliation><country wicri:rule="url">Oman</country><wicri:regionArea>Department of Medical Laboratory Science, Fenyang College of Shanxi Medical University, Fenyang 032200; Shanxi Medical University, First Affiliated Hospital of Shanxi Medical University, Taiyuan 030001, China. *Corresponding author</wicri:regionArea><wicri:noRegion>China. *Corresponding author</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2017">2017</date><idno type="RBID">pubmed:29382424</idno><idno type="pmid">29382424</idno><idno type="wicri:Area/PubMed/Corpus">000131</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000131</idno><idno type="wicri:Area/PubMed/Curation">000131</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000131</idno><idno type="wicri:Area/PubMed/Checkpoint">000135</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000135</idno><idno type="wicri:Area/Ncbi/Merge">000422</idno><idno type="wicri:Area/Ncbi/Curation">000422</idno><idno type="wicri:Area/Ncbi/Checkpoint">000422</idno><idno type="wicri:doubleKey">1007-8738:2017:Zhang Y:inhibition:of:autophagy</idno><idno type="wicri:Area/Main/Merge">000C84</idno><idno type="wicri:Area/Main/Curation">000C84</idno><idno type="wicri:Area/Main/Exploration">000C84</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">[Inhibition of autophagy initiation stage enhances camptothecin-induced apoptosis in NCI-H1975 cells].</title><author><name sortKey="Zhang, Yaping" sort="Zhang, Yaping" uniqKey="Zhang Y" first="Yaping" last="Zhang">Yaping Zhang</name><affiliation wicri:level="1"><nlm:affiliation>Department of Medical Laboratory Science, Fenyang College of Shanxi Medical University, Fenyang 032200; Shanxi Medical University, First Affiliated Hospital of Shanxi Medical University, Taiyuan 030001, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Medical Laboratory Science, Fenyang College of Shanxi Medical University, Fenyang 032200; Shanxi Medical University, First Affiliated Hospital of Shanxi Medical University, Taiyuan 030001</wicri:regionArea><wicri:noRegion>Taiyuan 030001</wicri:noRegion></affiliation></author><author><name sortKey="Cao, Li" sort="Cao, Li" uniqKey="Cao L" first="Li" last="Cao">Li Cao</name><affiliation wicri:level="1"><nlm:affiliation>Department of Medical Laboratory Science, Fenyang College of Shanxi Medical University, Fenyang 032200; Shanxi Medical University, First Affiliated Hospital of Shanxi Medical University, Taiyuan 030001, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Medical Laboratory Science, Fenyang College of Shanxi Medical University, Fenyang 032200; Shanxi Medical University, First Affiliated Hospital of Shanxi Medical University, Taiyuan 030001</wicri:regionArea><wicri:noRegion>Taiyuan 030001</wicri:noRegion></affiliation></author><author><name sortKey="Su, Qiang" sort="Su, Qiang" uniqKey="Su Q" first="Qiang" last="Su">Qiang Su</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Neurology, First Affiliated Hospital of Shanxi Medical University, Taiyuan 030001, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Neurology, First Affiliated Hospital of Shanxi Medical University, Taiyuan 030001</wicri:regionArea><wicri:noRegion>Taiyuan 030001</wicri:noRegion></affiliation></author><author><name sortKey="Cheng, Kai" sort="Cheng, Kai" uniqKey="Cheng K" first="Kai" last="Cheng">Kai Cheng</name><affiliation wicri:level="1"><nlm:affiliation>Department of Medical Laboratory Science, Fenyang College of Shanxi Medical University, Fenyang 032200, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Medical Laboratory Science, Fenyang College of Shanxi Medical University, Fenyang 032200</wicri:regionArea><wicri:noRegion>Fenyang 032200</wicri:noRegion></affiliation></author><author><name sortKey="Zhang, Xiaoyan" sort="Zhang, Xiaoyan" uniqKey="Zhang X" first="Xiaoyan" last="Zhang">Xiaoyan Zhang</name><affiliation wicri:level="1"><nlm:affiliation>Department of Medical Laboratory Science, Fenyang College of Shanxi Medical University, Fenyang 032200; Shanxi Medical University, First Affiliated Hospital of Shanxi Medical University, Taiyuan 030001, China. *Corresponding author, E-mail: 931326464@qq.com.</nlm:affiliation><country wicri:rule="url">Oman</country><wicri:regionArea>Department of Medical Laboratory Science, Fenyang College of Shanxi Medical University, Fenyang 032200; Shanxi Medical University, First Affiliated Hospital of Shanxi Medical University, Taiyuan 030001, China. *Corresponding author</wicri:regionArea><wicri:noRegion>China. *Corresponding author</wicri:noRegion></affiliation></author></analytic><series><title level="j">Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology</title><idno type="ISSN">1007-8738</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adenine (analogs & derivatives)</term><term>Adenine (pharmacology)</term><term>Apoptosis (drug effects)</term><term>Autophagy (drug effects)</term><term>Camptothecin (pharmacology)</term><term>Carcinoma, Non-Small-Cell Lung (drug therapy)</term><term>Carcinoma, Non-Small-Cell Lung (pathology)</term><term>Cell Line, Tumor</term><term>Cell Proliferation (drug effects)</term><term>Chloroquine (pharmacology)</term><term>Humans</term><term>Lung Neoplasms (drug therapy)</term><term>Lung Neoplasms (pathology)</term><term>Transforming Growth Factor beta1 (analysis)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adénine (analogues et dérivés)</term><term>Adénine (pharmacologie)</term><term>Apoptose ()</term><term>Autophagie ()</term><term>Camptothécine (pharmacologie)</term><term>Carcinome pulmonaire non à petites cellules (anatomopathologie)</term><term>Carcinome pulmonaire non à petites cellules (traitement médicamenteux)</term><term>Chloroquine (pharmacologie)</term><term>Facteur de croissance transformant bêta-1 (analyse)</term><term>Humains</term><term>Lignée cellulaire tumorale</term><term>Prolifération cellulaire ()</term><term>Tumeurs du poumon (anatomopathologie)</term><term>Tumeurs du poumon (traitement médicamenteux)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analogs & derivatives" xml:lang="en"><term>Adenine</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Transforming Growth Factor beta1</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Adenine</term><term>Camptothecin</term><term>Chloroquine</term></keywords><keywords scheme="MESH" qualifier="analogues et dérivés" xml:lang="fr"><term>Adénine</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Facteur de croissance transformant bêta-1</term></keywords><keywords scheme="MESH" qualifier="anatomopathologie" xml:lang="fr"><term>Carcinome pulmonaire non à petites cellules</term><term>Tumeurs du poumon</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Apoptosis</term><term>Autophagy</term><term>Cell Proliferation</term></keywords><keywords scheme="MESH" qualifier="drug therapy" xml:lang="en"><term>Carcinoma, Non-Small-Cell Lung</term><term>Lung Neoplasms</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Carcinoma, Non-Small-Cell Lung</term><term>Lung Neoplasms</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Adénine</term><term>Camptothécine</term><term>Chloroquine</term></keywords><keywords scheme="MESH" qualifier="traitement médicamenteux" xml:lang="fr"><term>Carcinome pulmonaire non à petites cellules</term><term>Tumeurs du poumon</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cell Line, Tumor</term><term>Humans</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Apoptose</term><term>Autophagie</term><term>Humains</term><term>Lignée cellulaire tumorale</term><term>Prolifération cellulaire</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Objective To explore the effect of autophagic inhibitors chloroquine (CQ) and 3-methyl adenine (3-MA) on apoptosis of non-small cell lung adenocarcinoma NCI-H1975 cells induced by camptothecin (CPT). Methods After NCI-H1975 cells were treated with CPT, cell proliferation was detected by CCK-8 assay, morphological changes of cells were observed by PI staining, and the apoptosis of NCI-H1975 cells was determined by flow cytometry. The levels of autophagy-and apoptosis-related proteins LC3I, LC3II, P62, caspase-3 and poly ADP-ribose polymerase (PARP) and transforming growth factor beta 1 (TGF-beta 1) were detected by Western blot analysis. Results After CPT treatment, the ratio of LC3II to LC3I was raised. The apoptotic protease caspase-3 and substrate PARP were obviously degraded, which could be enhanced by 3-MA but inhibited by CQ. It was also found that the intracellular TGF-beta 1 was reduced after CPT treatment. Conclusion Inhibition of autophagy initiation stage in NCI-H1975 cells can increase the sensitivity of cell apoptosis induced by CPT.</div></front></TEI><affiliations><list><country><li>Oman</li><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Zhang, Yaping" sort="Zhang, Yaping" uniqKey="Zhang Y" first="Yaping" last="Zhang">Yaping Zhang</name></noRegion><name sortKey="Cao, Li" sort="Cao, Li" uniqKey="Cao L" first="Li" last="Cao">Li Cao</name><name sortKey="Cheng, Kai" sort="Cheng, Kai" uniqKey="Cheng K" first="Kai" last="Cheng">Kai Cheng</name><name sortKey="Su, Qiang" sort="Su, Qiang" uniqKey="Su Q" first="Qiang" last="Su">Qiang Su</name></country><country name="Oman"><noRegion><name sortKey="Zhang, Xiaoyan" sort="Zhang, Xiaoyan" uniqKey="Zhang X" first="Xiaoyan" last="Zhang">Xiaoyan Zhang</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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